2 the university of aston in birmingham phenotypic and genotypic characterisation of clostridium difficile a thesis submitted by lauren michelle green bsc (hons) for. This thesis addresses the issue of the effects ofn^-benzyladenine (ba) levels and cold storage on genetic stability and morphological behavior of the strawberry plant upon transplantation in the studies described random amplified polymorphic dna (rapd) markers were used to determine if ba levels or cold storage lead to genetic changes. A thesis submitted in partial fulfillment of the requirements for the degree of master of science by keerti potluri and decreased rapd pcr failure rate. Random amplified polymorphic dna(rapd) and repetitive element pcr fingerprinting (gillings and holley, 1997 rodrigue et al, 1995 stubbs et al, 1994 olsen et al, 1993 lagotolla et al, 1996. J developing a procedure for establishing paternity in misumenoides /ormosipes using rapd pcr an honors thesis. Random amplified polymorphic dna (rapd) markers are dna fragments from pcr amplification of random segments of genomic dna. This thesis is my original extraction of dna and running of polymerase chain reaction genetic diversity of c kahawae using random amplified polymorphic dna. Use of rapd-pcr for genetic analysis of nicobari fowl of rapd-pcr for genetic analysis of nicobari dna fingerprinting and rapd markers phd thesis.
Use of rapd-pcr as a method to follow the progress of starter cultures in sauerkraut fermentation$ v plengvidhya, f breidt jr, hp fleming. Rapd: random amplified polymorphic dna initially, optimum pcr conditions for both rapd and issr were standardized with various quantities of template. An abstract of the thesis of hong xu for the degree of master of science in genetics presented on june 30, 1995 title: development of pcr-based markers for identif inz grape rootstocks. Markers derived from rapd-pcr have also been shown to be linked with zucchini yellow mosaic virus (zymv) resistance and powdery mildew resistance in squash (brown.
Application of polymerase chain reaction (pcr) using random amplified polymorphic dna (rapd) random amplified polymorphic dna pcr. Mphil thesis, quaid-i-azam university, islamabad, pakistan mare, l, l m dick and m l vanderwalt, 2001 characterization of south african isolates of salmonella.
However, over the last few years, polymerase chain reaction (pcr) technology has had a significant impact in almost all areas of molecular biology (saiki et al 1988) and modifications of the basic procedure have allowed the development of numerous assays for detecting variation at the nucleotide level (eg korner and livak 1989 newton et. Rapd-pcr was carried out in 25 µl reaction volume contains 125 µl master mix, 2 µl of single decamere primer, 4 µl of template dna and 65 µl distilled water 25 ng of dna template, 5 pmoles of primer, 01 mm of each dntps, 4 mm mgcl 2, 05 u of taq polymerase and master mix buffer solution. The rapd banding patterns of the 11 plant species are illustrated in figure 1 the 5 primers (a to e) used for rapd-pcr were able to amplify the dna from all the plant species studied the rapd profiles using all samples generated amplified products ranging from 307 to 1772 bp a total of 164 bands were observed for 11 plant species.
Typification of oenococcus oeni strains by multiplex rapd-pcr and study of population dynamics during malolactic fermentation phd thesis, tarragona. Thesis is accepted for the defence process originated from isolates and strains used for inoculation were typed by rapd-pcr as well.
Iranian journal of veterinary medicine ijvm (2016), 10 (1): 33 genetic variation among escherichia coli isolates from human and calves by using rapd pcr. Rapd-pcr analysis of staphylococcus aureus strains isolated from different sources. 232 afr j biotechnol table 1 optimization of the rapd-pcr reaction parameters for 1vitex pubescens, 2 nervilia aragoana 3. (ap-pcr) pcr with arbitrary primers 2003 pcr with arbitrary primers 4 rapd technology, step by step the random amplified polymorphic dna (rapd.
Please use this identifier to cite or link to this item:. Pcr protocol for fish and seafood authentication - msc thesis - free download as pdf file (pdf), text file (txt) or read online for free. Molecular characterization of listeria monocytogenes based on the pfge and rapd in korea rrna using multiplex-pcr we also compared rapd and pfge to. Optimization of dna concentration in rapd fingerprinting of phytophthora infestans - nesaratnam alwar - bachelor thesis - biology - genetics / gene technology. -title of thesis : the role of fish during the study using multiplex polymerase chain reaction using of random amplified polymorphic dna (rapd-pcr. The topic of this thesis is to differentiate and identify strains of the species lactobacillus buchneri, lactobacillus casei, lactobacillus plantarum, pediococcus pentosaceus and bifidobacterium bifidum the randomly amplified polymorphic dna-pcr (rapd-pcr) method is a suitable method for this study altogether 26 samples were analyzed.